• Western blot analysis of extracts of SKOV3 cells, using RSPO1 antibody (STJ110588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Anti-RSPO1 antibody (90-210) (STJ110588)
  • Western blot analysis of extracts of Rat pancreas, using RSPO1 antibody (STJ110588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of A-549 cells using RSPO1 Rabbit polyclonal antibody (STJ110588) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat ovary using RSPO1 rabbit polyclonal antibody (STJ110588) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using RSPO1 Rabbit polyclonal antibody (STJ110588) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A549 cells using RSPO1 rabbit polyclonal antibody (STJ110588) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat pancreas, using RSPO1 antibody (STJ110588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of C6 cells using RSPO1 rabbit polyclonal antibody (STJ110588) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of SKOV3 cells, using RSPO1 antibody (STJ110588) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RSPO1 antibody (90-210) (STJ110588)

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STJ110588

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RSPO1 (90-210) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RSPO1
Gene ID: 284654
Uniprot ID: RSPO1_HUMAN
Immunogen Region: 90-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human RSPO1 (NP_001033722.1).
Immunogen Sequence: DMNKCIKCKIEHCEACFSHN FCTKCKEGLYLHKGRCYPAC PEGSSAANGTMECSSPAQCE MSEWSPWGPCSKKQQLCGFR RGSEERTRRVLHAPVGDHAA CSDTKETRRCTVRRVPCPEG Q
Tissue Specificity Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
Function Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1.
Protein Name R-Spondin-1
Roof Plate-Specific Spondin-1
Hrspo1
Database Links Reactome: R-HSA-4641263
Cellular Localisation Secreted
Nucleus
Seems To Mainly Localize To Nucleoli
Alternative Antibody Names Anti-R-Spondin-1 antibody
Anti-Roof Plate-Specific Spondin-1 antibody
Anti-Hrspo1 antibody
Anti-RSPO1 antibody

Information sourced from Uniprot.org

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