Western blot analysis of extracts of various cell lines, using RRP8 antibody (STJ11103380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RRP8 antibody (100-200) (STJ11103380)

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STJ11103380
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRP8
Gene ID: 23378
Uniprot ID: RRP8_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RRP8 (NP_056139.1).
Immunogen Sequence: GPPCSDSEEEVERKKKCHKQ ALVGSDSAEDEKRKRKCQKH APINSAQHLDNVDQTGPKAW KGSTTNDPPKQSPGSTSPKP PHTLSRKQWRNRQKNKRRCK N
Function Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Protein Name Ribosomal Rna-Processing Protein 8
Cerebral Protein 1
Nucleomethylin
Database Links Reactome: R-HSA-427359
Cellular Localisation Nucleus
Nucleolus
Localizes At Rdna Locus
Alternative Antibody Names Anti-Ribosomal Rna-Processing Protein 8 antibody
Anti-Cerebral Protein 1 antibody
Anti-Nucleomethylin antibody
Anti-RRP8 antibody
Anti-KIAA0409 antibody
Anti-NML antibody
Anti-hucep-1 antibody

Information sourced from Uniprot.org

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