Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase RNF125 (131-180 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | RNF125 |
Gene ID: | 54941 |
Uniprot ID: | RN125_HUMAN |
Immunogen Region: | 131-180 aa |
Specificity: | TRAC-1 Polyclonal Antibody detects endogenous levels of TRAC-1 protein. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human RNF125 at the amino acid range 131-180 |
Post Translational Modifications | Autoubiquitinated, leading to its subsequent proteasomal degradation. |
Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation. |
Protein Name | E3 Ubiquitin-Protein Ligase Rnf125Ring Finger Protein 125T-Cell Ring Activation Protein 1Trac-1 |
Database Links | Reactome: R-HSA-936440 |
Cellular Localisation | Golgi Apparatus MembraneLipid-AnchorShows A Reticular Staining Pattern Within The Cell And Is Probably Expressed At Other Intracellular Membranes In Addition To The Golgi MembraneNot Detected At The Plasma Membrane |
Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf125 antibodyAnti-Ring Finger Protein 125 antibodyAnti-T-Cell Ring Activation Protein 1 antibodyAnti-Trac-1 antibodyAnti-RNF125 antibody |
Information sourced from Uniprot.org