| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RHOG |
| Gene ID: | 391 |
| Uniprot ID: | RHOG_HUMAN |
| Immunogen Region: | 50-150 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RHOG (NP_001656.2). |
| Immunogen Sequence: | TVNLNLWDTAGQEEYDRLRT LSYPQTNVFVICFSIASPPS YENVRHKWHPEVCHHCPDVP ILLVGTKKDLRAQPDTLRRL KEQGQAPITPQQGQALAKQI H |
| Post Translational Modifications | (Microbial infection) Glucosylated at Thr-35 by C.difficile toxin TcdB. (Microbial infection) Glycosylated (O-GlcNAcylated) at Thr-35 by C.novyi toxin TcdA. O-GlcNAcylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption. |
| Function | Plays a role in immunological synaptic F-actin density and architecture organization. Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity. Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Binds phospholipids in an activation-dependent manner.thereby acting as an anchor for other proteins to the plasma membrane (PM). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells. Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D. Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells. (Microbial infection) In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. |
| Protein Name | Rho-Related Gtp-Binding Protein Rhog |
| Database Links | Reactome: R-HSA-114604 Reactome: R-HSA-1257604 Reactome: R-HSA-2219530 Reactome: R-HSA-5625970 Reactome: R-HSA-6798695 Reactome: R-HSA-6811558 Reactome: R-HSA-9013408 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Cytoplasmic Side |
| Alternative Antibody Names | Anti-Rho-Related Gtp-Binding Protein Rhog antibody Anti-RHOG antibody Anti-ARHG antibody |
Information sourced from Uniprot.org