| Host: | Mouse |
| Applications: | IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Transforming protein RhoA is suitable for use in Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | Mix |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RHOA |
| Gene ID: | 387 |
| Uniprot ID: | RHOA_HUMAN |
| Specificity: | This antibody detects endogenous levels of Rho A at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from the human Rho A |
| Function | Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation.promotes KCNA2 endocytosis. Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center. May be an activator of PLCE1. In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets. When activated by DAAM1 may signal centrosome maturation and chromosomal segregation during cell division. May also be involved in contractile ring formation during cytokinesis. (Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague. |
| Protein Name | Transforming Protein Rhoa Rho Cdna Clone 12 H12 |
| Database Links | Reactome: R-HSA-114604 Reactome: R-HSA-193634 Reactome: R-HSA-198203 Reactome: R-HSA-209563 Reactome: R-HSA-2173791 Reactome: R-HSA-392451 Reactome: R-HSA-3928662 Reactome: R-HSA-3928663 Reactome: R-HSA-4086400 Reactome: R-HSA-416482 Reactome: R-HSA-416550 Reactome: R-HSA-416572 Reactome: R-HSA-4420097 Reactome: R-HSA-5625740 Reactome: R-HSA-5625900 Reactome: R-HSA-5625970 Reactome: R-HSA-5627117 Reactome: R-HSA-5663220 Reactome: R-HSA-5666185 Reactome: R-HSA-5689896 Reactome: R-HSA-6785631 Reactome: R-HSA-6798695 Reactome: R-HSA-8849471 Reactome: R-HSA-8980692 Reactome: R-HSA-8985586 Reactome: R-HSA-9013106 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Cytoplasmic Side Cytoplasm Cytoskeleton Cleavage Furrow Cell Cortex Midbody Cell Projection Lamellipodium Dendrite Nucleus Localized To Cell-Cell Contacts In Calcium-Treated Keratinocytes Translocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner Localizes To The Equatorial Cell Cortex (At The Site Of The Presumptive Furrow) In Early Anaphase In An Activated Form And In A Myosin- And Actin-Independent Manner Colocalizes With Kank1 At The Contractile Ring Colocalizes With Daam1 And Kank1 Around Centrosomes |
| Alternative Antibody Names | Anti-Transforming Protein Rhoa antibody Anti-Rho Cdna Clone 12 antibody Anti-H12 antibody Anti-RHOA antibody Anti-ARH12 antibody Anti-ARHA antibody Anti-RHO12 antibody |
Information sourced from Uniprot.org