• Western blot analysis of extracts of various cell lines, using RGS8 antibody (STJ112028) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RGS8 antibody (1-180) (STJ112028)

SKU:
STJ112028

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RGS8 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RGS8
Gene ID: 85397
Uniprot ID: RGS8_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human RGS8 (NP_001095920.1).
Immunogen Sequence: MAALLMPRRNKGMRTRLGCL SHKSDSCSDFTAILPDKPNR ALKRLSTEEATRWADSFDVL LSHKYGVAAFRAFLKTEFSE ENLEFWLACEEFKKTRSTAK LVSKAHRIFEEFVDVQAPRE VNIDFQTREATRKNLQEPSL TCFDQAQGKVHSLMEKDSYP RFLRSKMYLDLLSQSQRRLS
Function Regulates G protein-coupled receptor signaling cascades, including signaling via muscarinic acetylcholine receptor CHRM2 and dopamine receptor DRD2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Modulates the activity of potassium channels that are activated in response to DRD2 and CHRM2 signaling.
Protein Name Regulator Of G-Protein Signaling 8
Rgs8
Database Links Reactome: R-HSA-418594
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Perikaryon
Cell Projection
Dendrite
Nucleus
Detected In Purkinje Cell Soma And Dendrites
Associated With Purkinje Cell Membranes
Not Detected In Purkinje Cell Nuclei
Detected In The Nucleus After Heterologous Expression
Recruited To The Cell Membrane In The Presence Of Gnao1
Alternative Antibody Names Anti-Regulator Of G-Protein Signaling 8 antibody
Anti-Rgs8 antibody
Anti-RGS8 antibody

Information sourced from Uniprot.org

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