| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RFFL (1-190) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RFFL |
| Gene ID: |
117584 |
| Uniprot ID: |
RFFL_HUMAN |
| Immunogen Region: |
1-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RFFL (NP_001017368.1). |
| Immunogen Sequence: |
MWATCCNWFCLDGQPEEVPP PQGARMQAYSNPGYSSFPSP TGLEPSCKSCGAHFANTARK QTCLDCKKNFCMTCSSQVGN GPRLCLLCQRFRATAFQREE LMKMKVKDLRDYLSLHDIST EMCREKEELVLLVLGQQPVI SQEDRTRASTLSPDFPEQQA FLTQPHSSMVPPTSPNLPSS SAQATSVPPA |
| Tissue Specificity | Ubiquitous. Detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. |
| Post Translational Modifications | Autoubiquitinated. Palmitoylated. Undergoes caspase-mediated cleavage upon death-receptor activation, by TNFSF10 for instance. May be mediated by the caspases CASP8 and CASP10 in a negative feedback loop. |
| Function | E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling. |
| Protein Name | E3 Ubiquitin-Protein Ligase RififylinCaspase Regulator Carp2Caspases-8 And -10-Associated Ring Finger Protein 2Carp-2Fyve-Ring Finger Protein SakuraFringRing Finger And Fyve-Like Domain-Containing Protein 1Ring Finger Protein 189Ring Finger Protein 34-LikeRing-Type E3 Ubiquitin Transferase Rififylin |
| Database Links | Reactome: R-HSA-6804757 |
| Cellular Localisation | CytoplasmCytosolCell MembranePeripheral Membrane ProteinRecycling Endosome MembraneThe Fyve-Type Zinc Finger May Mediate Phosphatidylinositol Phosphate-Binding And Control Subcellular Localization |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rififylin antibodyAnti-Caspase Regulator Carp2 antibodyAnti-Caspases-8 And -10-Associated Ring Finger Protein 2 antibodyAnti-Carp-2 antibodyAnti-Fyve-Ring Finger Protein Sakura antibodyAnti-Fring antibodyAnti-Ring Finger And Fyve-Like Domain-Containing Protein 1 antibodyAnti-Ring Finger Protein 189 antibodyAnti-Ring Finger Protein 34-Like antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rififylin antibodyAnti-RFFL antibodyAnti-RNF189 antibodyAnti-RNF34L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
