| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Ran GTPase-activating protein 1 is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RANGAP1 |
| Gene ID: | 5905 |
| Uniprot ID: | RAGP1_HUMAN |
| Specificity: | RAGP1 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from part of the human protein |
| Post Translational Modifications | Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBE2I. Desumoylated by HINT1. |
| Function | GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export. |
| Protein Name | Ran Gtpase-Activating Protein 1 Rangap1 |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-165054 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-4615885 Reactome: R-HSA-5663220 Reactome: R-HSA-68877 Reactome: R-HSA-9615933 Reactome: R-HSA-9648025 Reactome: R-HSA-9793242 |
| Cellular Localisation | Cytoplasm Nucleus Nucleoplasm Nucleus Envelope Chromosome Centromere Kinetochore Cytoskeleton Spindle Cytoplasmic During Interphase Detected At The Nuclear Envelope During Interphase Targeted To The Nuclear Pores After Sumoylation During Mitosis Associates With Mitotic Spindles But Is Essentially Not Detected At The Spindle Poles Association With Kinetochores Appears Soon After Nuclear Envelope Breakdown And Persists Until Late Anaphase Mitotic Location Also Requires Sumoylation |
| Alternative Antibody Names | Anti-Ran Gtpase-Activating Protein 1 antibody Anti-Rangap1 antibody Anti-RANGAP1 antibody Anti-KIAA1835 antibody Anti-SD antibody |
Information sourced from Uniprot.org