• Western blot analysis of extracts of various cell lines, using RAB3GAP1 antibody (STJ11100534) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.

Anti-RAB3GAP1 antibody (STJ11100534)

SKU:
STJ11100534
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against RAB3GAP1.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: RB3GP_HUMAN
Gene ID: 22930
Gene Symbol: RAB3GAP1
Immunogen: Recombinant protein of human RAB3GAP1.
Background This gene encodes the catalytic subunit of a Rab GTPase activating protein. The encoded protein forms a heterodimer with a non-catalytic subunit to specifically regulate the activity of members of the Rab3 subfamily of small G proteins. This protein mediates the hydrolysis of GTP bound Rab3 to the GDP bound form. Mutations in this gene are associated with Warburg micro syndrome. Alternate splicing results in multiple transcript variants.
Tissue Specificity Ubiquitous.
Function Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
Database Links Reactome: R-HSA-6811436
Cellular Localisation Cytoplasm
In Neurons
It Is Enriched In The Synaptic Soluble Fraction
Protein Name Rab3 Gtpase-Activating Protein Catalytic Subunit
Rab3 Gtpase-Activating Protein 130 Kda Subunit
Rab3-Gap P130
Rab3-Gap
Alternative Names Anti-Rab3 Gtpase-Activating Protein Catalytic Subunit antibody
Anti-Rab3 Gtpase-Activating Protein 130 Kda Subunit antibody
Anti-Rab3-Gap P130 antibody
Anti-Rab3-Gap antibody
Anti-RAB3GAP1 antibody
Anti-KIAA0066 antibody
Anti-RAB3GAP antibody
6 months standard