| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PUS10 (290-529) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PUS10 |
| Gene ID: |
150962 |
| Uniprot ID: |
PUS10_HUMAN |
| Immunogen Region: |
290-529 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 290-529 of human PUS10 (NP_653310.2). |
| Immunogen Sequence: |
VAGRYNKYSRNLPQTPWIID GERKLESSVEELISDHLLAV FKAESFNFSSSGREDVDVRT LGNGRPFAIELVNPHRVHFT SQEIKELQQKINNSSNKIQV RDLQLVTREAIGHMKEGEEE KTKTYSALIWTNKAIQKKDI EFLNDIKDLKIDQKTPLRVL HRRPLAVRARVIHFMETQYV DEHHFRLHLKTQAGTYIKEF VHGDFGRTKPNIGSLMNVTA DILELDVESVDVDWPPALD |
| Post Translational Modifications | Proteolytically cleaved during TRAIL-induced cell death. Cleaved, in vitro, either by caspase-3 (CASP3) or caspase-8 (CASP8). |
| Function | Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. Does not show tRNA pseudouridylate synthase activity in the nucleus. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. |
| Protein Name | Trna Pseudouridine Synthase Pus10Hup10Coiled-Coil Domain-Containing Protein 139Trna Pseudouridine 55 SynthasePsi55 SynthaseTrna Pseudouridylate SynthaseTrna-Uridine Isomerase |
| Cellular Localisation | NucleusCytoplasmMitochondrionLocalizes Mainly In The Nucleus (Probable)Trna Pseudouridylate Synthase Activity Is Restricted To The CytoplasmTranslocates From Nucleus To Mitochondria During Trail-Induced Apoptosis |
| Alternative Antibody Names | Anti-Trna Pseudouridine Synthase Pus10 antibodyAnti-Hup10 antibodyAnti-Coiled-Coil Domain-Containing Protein 139 antibodyAnti-Trna Pseudouridine 55 Synthase antibodyAnti-Psi55 Synthase antibodyAnti-Trna Pseudouridylate Synthase antibodyAnti-Trna-Uridine Isomerase antibodyAnti-PUS10 antibodyAnti-CCDC139 antibodyAnti-DOBI antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
