| Host: | Mouse |
| Applications: | IHC/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-5 AMP-activated protein kinase subunit gamma-1 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 3G12 |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC-P 1:50-200 WB 1:1000-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRKAG1 |
| Gene ID: | 5571 |
| Uniprot ID: | AAKG1_HUMAN |
| Specificity: | This antibody detects endogenous levels of AMPK Gamma 1 at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from the human AMPK Gamma 1 |
| Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Gamma-1 Ampk Gamma1 Ampk Subunit Gamma-1 Ampkg |
| Database Links | Reactome: R-HSA-1445148 Reactome: R-HSA-1632852 Reactome: R-HSA-2151209 Reactome: R-HSA-380972 Reactome: R-HSA-5628897 Reactome: R-HSA-6804756 Reactome: R-HSA-9613354 Reactome: R-HSA-9619483 |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Gamma-1 antibody Anti-Ampk Gamma1 antibody Anti-Ampk Subunit Gamma-1 antibody Anti-Ampkg antibody Anti-PRKAG1 antibody |
Information sourced from Uniprot.org