| Host: | Rabbit |
| Applications: | WB/IHC-F/IHC-P/ICC/IF/IP |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-cAMP Protein Kinase Catalytic Subunit (319-332 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality : | Monoclonal |
| Clone ID : | R07-8H3 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/ml |
| Dilution Range: | WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:200 IP 1:20 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | PRKACA |
| Gene ID: | 5566 |
| Uniprot ID: | KAPCA_HUMAN |
| Immunogen Region: | 319-332 aa |
| Immunogen: | A synthetic peptide of human cAMP Protein Kinase Catalytic subunit |
| Tissue Specificity | Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids. |
| Post Translational Modifications | Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity. Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR.this increases catalytic efficiency. Asn-3 is partially deaminated to Asp-3 giving rise to 2 major isoelectric variants, called CB and CA respectively. When myristoylated, Ser-11 is autophosphorylated probably in conjunction with deamidation of Asn-3. |
| Function | Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in chondrogenesis by mediating phosphorylation of SOX9. Involved in the regulation of platelets in response to thrombin and collagen.maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Required for phosphorylation of GLI transcription factors which inhibits them and prevents transcriptional activation of Hedgehog signaling pathway target genes. GLI transcription factor phosphorylation is inhibited by interaction of PRKACA with SMO which sequesters PRKACA at the cell membrane. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis most probably through the regulation of OFD1 in ciliogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Phosphorylates HSF1.this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR. Isoform 2: Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. |
| Protein Name | Camp-Dependent Protein Kinase Catalytic Subunit Alpha Pka C-Alpha |
| Database Links | Reactome: R-HSA-111931 Reactome: R-HSA-163358 Reactome: R-HSA-163560 Reactome: R-HSA-163615 Reactome: R-HSA-164378 Reactome: R-HSA-180024 Reactome: R-HSA-2565942 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-381676 Reactome: R-HSA-392517 Reactome: R-HSA-422356 Reactome: R-HSA-432040 Reactome: R-HSA-4420097 Reactome: R-HSA-442720 Reactome: R-HSA-512988 Reactome: R-HSA-5578775 Reactome: R-HSA-5610780 Reactome: R-HSA-5610783 Reactome: R-HSA-5610785 Reactome: R-HSA-5610787 Reactome: R-HSA-5620912 Reactome: R-HSA-5621575 Reactome: R-HSA-5687128 Reactome: R-HSA-8853659 Reactome: R-HSA-8854518 Reactome: R-HSA-8963896 Reactome: R-HSA-9010642 Reactome: R-HSA-9022535 Reactome: R-HSA-9022692 Reactome: R-HSA-9634597 Reactome: R-HSA-9634600 Reactome: R-HSA-9660821 Reactome: R-HSA-9664323 Reactome: R-HSA-983231 Reactome: R-HSA-9837999 |
| Cellular Localisation | Cytoplasm Cell Membrane Membrane Lipid-Anchor Nucleus Mitochondrion Translocates Into The Nucleus (Monomeric Catalytic Subunit) The Inactive Holoenzyme Is Found In The Cytoplasm Distributed Throughout The Cytoplasm In Meiotically Incompetent Oocytes Associated To Mitochondrion As Meiotic Competence Is Acquired Aggregates Around The Germinal Vesicles (Gv) At The Immature Gv Stage Oocytes Colocalizes With Hsf1 In Nuclear Stress Bodies (Nsbs) Upon Heat Shock Recruited To The Cell Membrane Through Interaction With Smo Isoform 2: Cell Projection Cilium Flagellum Cytoplasmic Vesicle Secretory Vesicle Acrosome Expressed In The Midpiece Region Of The Sperm Flagellum Colocalizes With Mroh2b And Tcp11 On The Acrosome And Tail Regions In Round Spermatids And Spermatozoa Regardless Of The Capacitation Status Of The Sperm |
| Alternative Antibody Names | Anti-Camp-Dependent Protein Kinase Catalytic Subunit Alpha antibody Anti-Pka C-Alpha antibody Anti-PRKACA antibody Anti-PKACA antibody |
Information sourced from Uniprot.org