• Western blot analysis of extracts of various cell lines, using PRAP1 antibody (STJ113585) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using PRAP1 Rabbit polyclonal antibody (STJ113585) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PRAP1 antibody (1-151) (STJ113585)

SKU:
STJ113585

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRAP1 (1-151) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRAP1
Gene ID: 118471
Uniprot ID: PRAP1_HUMAN
Immunogen Region: 1-151
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human PRAP1 (NP_660203.3).
Immunogen Sequence: MRRLLLVTSLVVVLLWEAGA VPAPKVPIKMQVKHWPSEQD PEKAWGARVVEPPEKDDQLV VLFPVQKPKLLTTEEKPRGQ GRGPILPGTKAWMETEDTLG HVLSPEPDHDSLYHPPPEED QGEERPRLWVMPNHQVLLGP EEDQDHIYHPQ
Tissue Specificity Highly expressed in the intestinal epithelial cells (at protein level). Abundantly expressed in the epithelial cells of the liver, kidney and cervix. Significantly down-regulated in hepatocellular carcinoma and right colon adenocarcinoma compared with the respective adjacent normal tissues. Expressed in epididymis (at protein level).
Function Lipid-binding protein which promotes lipid absorption by facilitating MTTP-mediated lipid transfer (mainly triglycerides and phospholipids) and MTTP-mediated apoB lipoprotein assembly and secretion. Protects the gastrointestinal epithelium from irradiation-induced apoptosis. May play an important role in maintaining normal growth homeostasis in epithelial cells. Involved in p53/TP53-dependent cell survival after DNA damage. May down-regulate the expression of MAD1L1 and exert a suppressive role in mitotic spindle assembly checkpoint in hepatocellular carcinomas.
Protein Name Proline-Rich Acidic Protein 1
Epididymis Tissue Protein Li 178
Uterine-Specific Proline-Rich Acidic Protein
Cellular Localisation Secreted
Endoplasmic Reticulum
Alternative Antibody Names Anti-Proline-Rich Acidic Protein 1 antibody
Anti-Epididymis Tissue Protein Li 178 antibody
Anti-Uterine-Specific Proline-Rich Acidic Protein antibody
Anti-PRAP1 antibody
Anti-UPA antibody
Anti-UNQ608 antibody
Anti-PRO1195 antibody

Information sourced from Uniprot.org

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