• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g PPP1R12A antibody (STJ113547). Western blot was performed from the immunoprecipitate using PPP1R12A antibody (STJ113547) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using PPP1R12A Rabbit polyclonal antibody (STJ113547) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using PPP1R12A Rabbit polyclonal antibody (STJ113547) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PPP1R12A Rabbit polyclonal antibody (STJ113547) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PPP1R12A Rabbit polyclonal antibody (STJ113547) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PPP1R12A Rabbit polyclonal antibody (STJ113547) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PPP1R12A antibody (1-200) (STJ113547)

SKU:
STJ113547

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1R12A (1-200) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R12A
Gene ID: 4659
Uniprot ID: MYPT1_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PPP1R12A (NP_002471.1).
Immunogen Sequence: MKMADAKQKRNEQLKRWIGS ETDLEPPVVKRQKTKVKFDD GAVFLAACSSGDTDEVLKLL HRGADINYANVDGLTALHQA CIDDNVDMVKFLVENGANIN QPDNEGWIPLHAAASCGYLD IAEFLIGQGAHVGAVNSEGD TPLDIAEEEAMEELLQNEVN RQGVDIEAARKEEERIMLRD ARQWLNSGHINDVRHAKSGG
Tissue Specificity Expressed in striated muscles, specifically in type 2a fibers (at protein level).
Post Translational Modifications Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK.may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.
Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Protein Name Protein Phosphatase 1 Regulatory Subunit 12a
Myosin Phosphatase-Targeting Subunit 1
Myosin Phosphatase Target Subunit 1
Protein Phosphatase Myosin-Binding Subunit
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Cellular Localisation Cytoplasm
Cytoskeleton
Stress Fiber
Also Along Actomyosin Filaments
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 12a antibody
Anti-Myosin Phosphatase-Targeting Subunit 1 antibody
Anti-Myosin Phosphatase Target Subunit 1 antibody
Anti-Protein Phosphatase Myosin-Binding Subunit antibody
Anti-PPP1R12A antibody
Anti-MBS antibody
Anti-MYPT1 antibody

Information sourced from Uniprot.org

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