• Western blot analysis of extracts of various cell lines, using PPCS antibody (STJ114709) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-PPCS antibody (1-311) (STJ114709)

SKU:
STJ114709

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPCS (1-311) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPCS
Gene ID: 79717
Uniprot ID: PPCS_HUMAN
Immunogen Region: 1-311
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human PPCS (NP_078940.2).
Immunogen Sequence: MAEMDPVAEFPQPPGAARWA EVMARFAARLGAQGRRVVLV TSGGTKVPLEARPVRFLDNF SSGRRGATSAEAFLAAGYGV LFLYRARSAFPYAHRFPPQT WLSALRPSGPALSGLLSLEA EENALPGFAEALRSYQEAAA AGTFLAVEFTTLADYLHLLQ AAAQALNPLGPSAMFYLAAA VSDFYVPVSEMPEHKIQSSG GPLQITMKMVPKLLSPLVKD WAPKAFIISFKLETDPAIV
Function Catalyzes the second step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. Has a preference for ATP over CTP as a cosubstrate.
Protein Name Phosphopantothenate--Cysteine Ligase
Phosphopantothenoylcysteine Synthetase
Ppc Synthetase
Database Links Reactome: R-HSA-196783
Alternative Antibody Names Anti-Phosphopantothenate--Cysteine Ligase antibody
Anti-Phosphopantothenoylcysteine Synthetase antibody
Anti-Ppc Synthetase antibody
Anti-PPCS antibody
Anti-COAB antibody

Information sourced from Uniprot.org

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