• Western blot analysis of extracts of various cell lines, using POU4F3 antibody (STJ115984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-POU4F3 antibody (1-180) (STJ115984)

SKU:
STJ115984

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POU4F3 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU4F3
Gene ID: 5459
Uniprot ID: PO4F3_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human POU4F3 (NP_002691.1).
Immunogen Sequence: MMAMNSKQPFGMHPVLQEPK FSSLHSGSEAMRRVCLPAPQ LQGNIFGSFDESLLARAEAL AAVDIVSHGKNHPFKPDATY HTMSSVPCTSTSSTVPISHP AALTSHPHHAVHQGLEGDLL EHISPTLSVSGLGAPEHSVM PAQIHPHHLGAMGHLHQAMG MSHPHTVAPHSAMPACLSDV
Tissue Specificity Brain. Seems to be specific to the retina.
Function Acts as a transcriptional activator. Acts by binding to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Involved in the auditory system development, required for terminal differentiation of hair cells in the inner ear.
Protein Name Pou Domain - Class 4 - Transcription Factor 3
Brain-Specific Homeobox/Pou Domain Protein 3c
Brain-3c
Brn-3c
Cellular Localisation Nucleus
Cytoplasm
Preferentially Localized In The Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 4 - Transcription Factor 3 antibody
Anti-Brain-Specific Homeobox/Pou Domain Protein 3c antibody
Anti-Brain-3c antibody
Anti-Brn-3c antibody
Anti-POU4F3 antibody
Anti-BRN3C antibody

Information sourced from Uniprot.org

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