| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLR3A |
| Gene ID: |
11128 |
| Uniprot ID: |
RPC1_HUMAN |
| Immunogen Region: |
1-200 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR3A (NP_008986.2). |
| Immunogen Sequence: |
MVKEQFRETDVAKKISHICF GMKSPEEMRQQAHIQVVSKN LYSQDNQHAPLLYGVLDHRM GTSEKDRPCETCGKNLADCL GHYGYIDLELPCFHVGYFRA VIGILQMICKTCCHIMLSQE EKKQFLDYLKRPGLTYLQKR GLKKKISDKCRKKNICHHCG AFNGTVKKCGLLKIIHEKYK TNKKVVDPIVSNFLQSFETA |
| Tissue Specificity | Expressed in the brain, in the cortex and the white matter (at protein level). |
| Function | Catalytic core component of RNA polymerase III (Pol III), a DNA-dependent RNA polymerase which synthesizes small non-coding RNAs using the four ribonucleoside triphosphates as substrates. Synthesizes 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Pol III-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol III is recruited to DNA promoters type I, II or III with the help of general transcription factors and other specific initiation factors. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA. Forms Pol III active center together with the second largest subunit POLR3B/RPC2. Appends one nucleotide at a time to the 3' end of the nascent RNA, with POLR3A/RPC1 contributing a Mg(2+)-coordinating DxDGD motif, and POLR3B/RPC2 participating in the coordination of a second Mg(2+) ion and providing lysine residues believed to facilitate Watson-Crick base pairing between the incoming nucleotide and template base. Typically, Mg(2+) ions direct a 5' nucleoside triphosphate to form a phosphodiester bond with the 3' hydroxyl of the preceding nucleotide of the nascent RNA, with the elimination of pyrophosphate. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway. |
| Protein Name | Dna-Directed Rna Polymerase Iii Subunit Rpc1Rna Polymerase Iii Subunit C1Dna-Directed Rna Polymerase Iii Largest SubunitDna-Directed Rna Polymerase Iii Subunit ARna Polymerase Iii 155 Kda SubunitRpc155Rna Polymerase Iii Subunit C160 |
| Database Links | Reactome: R-HSA-1834949Reactome: R-HSA-73780Reactome: R-HSA-73980Reactome: R-HSA-749476Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071 |
| Cellular Localisation | NucleusCytoplasmCytosol |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase Iii Subunit Rpc1 antibodyAnti-Rna Polymerase Iii Subunit C1 antibodyAnti-Dna-Directed Rna Polymerase Iii Largest Subunit antibodyAnti-Dna-Directed Rna Polymerase Iii Subunit A antibodyAnti-Rna Polymerase Iii 155 Kda Subunit antibodyAnti-Rpc155 antibodyAnti-Rna Polymerase Iii Subunit C160 antibodyAnti-POLR3A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
