• Western blot analysis of extracts of various cell lines, using POLR2K antibody (STJ111878) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-POLR2K antibody (1-58) (STJ111878)

SKU:
STJ111878

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLR2K (1-58) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR2K
Gene ID: 5440
Uniprot ID: RPAB4_HUMAN
Immunogen Region: 1-58
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-58 of human POLR2K (NP_005025.1).
Immunogen Sequence: MDTQKDVQPPKQQPMIYICG ECHTENEIKSRDPIRCRECG YRIMYKKRTKRLVVFDAR
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.
Protein Name Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc4
Rna Polymerases I - Ii - And Iii Subunit Abc4
Abc10-Alpha
Dna-Directed Rna Polymerase Ii Subunit K
Rna Polymerase Ii 7.0 Kda Subunit
Rpb7.0
Rpb10alpha
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-168325
Reactome: R-HSA-1834949
Reactome: R-HSA-203927
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5578749
Reactome: R-HSA-5601884
Reactome: R-HSA-5617472
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-6803529
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72203
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73780
Reactome: R-HSA-73863
Reactome: R-HSA-73980
Reactome: R-HSA-749476
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-76061
Reactome: R-HSA-76066
Reactome: R-HSA-76071
Reactome: R-HSA-77075
Reactome: R-HSA-8851708
Reactome: R-HSA-9018519
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc4 antibody
Anti-Rna Polymerases I - Ii - And Iii Subunit Abc4 antibody
Anti-Abc10-Alpha antibody
Anti-Dna-Directed Rna Polymerase Ii Subunit K antibody
Anti-Rna Polymerase Ii 7.0 Kda Subunit antibody
Anti-Rpb7.0 antibody
Anti-Rpb10alpha antibody
Anti-POLR2K antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance