• Western blot analysis of extracts of various cell lines, using PLK5 antibody (STJ116652) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PLK5 antibody (1-336) (STJ116652)

SKU:
STJ116652

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLK5 (1-336) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLK5
Gene ID: 126520
Uniprot ID: PLK5_HUMAN
Immunogen Region: 1-336
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human PLK5 (NP_001230008.1).
Immunogen Sequence: MYTVLTGTPPFMASPLSEMY QNIREGHYPEPAHLSANARR LIVHLLAPNPAERPSLDHLL QDDFFTQGFTPDRLPAHSCH SPPIFAIPPPLGRIFRKVGQ RLLTQCRPPCPFTPKEASGP GEGGPDPDSMEWDGESSLSA KEVPCLEGPIHLVAQGTLQS DLAGPEGSRRPEVEAALRHL QLCLDVGPPATQDPLGEQQP ILWAPKWVDYSSKYGFGYQL LDGGRTGRHPHGPATPRRE
Tissue Specificity Expressed in the brain, neurons and glial cells. Also expressed in highly differentiated cells, such as the serous acini in the parotid gland, distal and proximal tubules of the kidney, tubules of the seminal gland, Kupffer cells and some hepatocytes in the liver, and some cells in the germinal center of lymph nodes (at protein level).
Function Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation.
Protein Name Inactive Serine/Threonine-Protein Kinase Plk5
Polo-Like Kinase 5
Plk-5
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Alternative Antibody Names Anti-Inactive Serine/Threonine-Protein Kinase Plk5 antibody
Anti-Polo-Like Kinase 5 antibody
Anti-Plk-5 antibody
Anti-PLK5 antibody
Anti-PLK5P antibody
Anti-FG060302 antibody

Information sourced from Uniprot.org

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