• Western blot analysis of extracts of U-87MG cells, using PIGZ antibody (STJ116636) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PIGZ antibody (435-579) (STJ116636)

SKU:
STJ116636

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIGZ (435-579) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGZ
Gene ID: 80235
Uniprot ID: PIGZ_HUMAN
Immunogen Region: 435-579
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 435-579 of human PIGZ (NP_079439.2).
Immunogen Sequence: LVPGLEYLEQVVHAPVLPST PTHYTLLFTHTYMPPRHLLH LPGLGAPVEVVDMGGTEDWA LCQTLKSFTRQPACQVAGGP WLCRLFVVTPGTTRRAVEKC SFPFKNETLLFPHLTLEDPP ALSSLLSGAWRDHLSLHIVE LGEET
Tissue Specificity Widely expressed at low level, with highest level in brain and colon.
Function Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is facultative and only scarcely detected, suggesting that it only exists in some tissues.
Protein Name Gpi Mannosyltransferase 4
Gpi Mannosyltransferase Iv
Gpi-Mt-Iv
Phosphatidylinositol-Glycan Biosynthesis Class Z Protein
Pig-Z
Smp3 Homolog
Hsmp3
Database Links Reactome: R-HSA-162710
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Gpi Mannosyltransferase 4 antibody
Anti-Gpi Mannosyltransferase Iv antibody
Anti-Gpi-Mt-Iv antibody
Anti-Phosphatidylinositol-Glycan Biosynthesis Class Z Protein antibody
Anti-Pig-Z antibody
Anti-Smp3 Homolog antibody
Anti-Hsmp3 antibody
Anti-PIGZ antibody
Anti-SMP3 antibody

Information sourced from Uniprot.org

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