| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S1MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PIAS2 |
| Gene ID: | 9063 |
| Uniprot ID: | PIAS2_HUMAN |
| Immunogen Region: | 50-150 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PIAS2 (NP_004662.2). |
| Immunogen Sequence: | SPAVQIKIRELYRRRYPRTL EGLSDLSTIKSSVFSLDGGS SPVEPDLAVAGIHSLPSTSV TPHSPSSPVGSVLLQDTKPT FEMQQPSPPIPPVHPDVQLK N |
| Tissue Specificity | Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs. |
| Post Translational Modifications | Sumoylated. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. |
| Protein Name | E3 Sumo-Protein Ligase Pias2 Androgen Receptor-Interacting Protein 3 Arip3 Dab2-Interacting Protein Dip E3 Sumo-Protein Transferase Pias2 Msx-Interacting Zinc Finger Protein Miz1 Pias-Ny Protein Protein Inhibitor Of Activated Stat X Protein Inhibitor Of Activated Stat2 |
| Database Links | Reactome: R-HSA-3108214 O75928-1 Reactome: R-HSA-3232118 O75928-1 Reactome: R-HSA-3232142 O75928-1 Reactome: R-HSA-3899300 O75928-1 Reactome: R-HSA-4090294 Reactome: R-HSA-4551638 O75928-2 Reactome: R-HSA-5617472 |
| Cellular Localisation | Nucleus Speckle Nucleus Pml Body Colocalizes At Least Partially With Promyelocytic Leukemia Nuclear Bodies (Pml Nbs) Colocalizes With Sumo1 In Nuclear Granules |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias2 antibody Anti-Androgen Receptor-Interacting Protein 3 antibody Anti-Arip3 antibody Anti-Dab2-Interacting Protein antibody Anti-Dip antibody Anti-E3 Sumo-Protein Transferase Pias2 antibody Anti-Msx-Interacting Zinc Finger Protein antibody Anti-Miz1 antibody Anti-Pias-Ny Protein antibody Anti-Protein Inhibitor Of Activated Stat X antibody Anti-Protein Inhibitor Of Activated Stat2 antibody Anti-PIAS2 antibody Anti-PIASX antibody |
Information sourced from Uniprot.org