• Western blot analysis of extracts from A549 cells untreated and treated with Epidermal Growth Factor (10ng/ml, 15min) , using Phospho-Tyrosine Mouse mAb.The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.

Anti-Phospho-Tyrosine antibody [7B4-D9-H10] (STJ99257)

SKU:
STJ99257

Current Stock:
Host: Mouse
Applications: WB
Reactivity: All
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Mouse monoclonal antibody anti-Phospho-Tyrosine is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 7B4-D9-H10
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was isolated from ascitic fluid by immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: Phospho-Tyrosine monoclonal antibody (Tyrosine) binds to endogenous Tyrosine only when phosphorylated at.
Immunogen: Phospho-tyrosine containing peptides.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance