| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Alpha-synuclein-Tyr125 (91-140 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SNCA |
| Gene ID: | 6622 |
| Uniprot ID: | SYUA_HUMAN |
| Immunogen Region: | 91-140 aa |
| Specificity: | Phospho-Synuclein-Alpha (Y125) Polyclonal Antibody detects endogenous levels of Synuclein-Alpha protein only when phosphorylated at Y125. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Synuclein-alpha around the phosphorylation site of Tyr125 at the amino acid range 91-140 |
| Post Translational Modifications | Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
| Protein Name | Alpha-Synuclein Non-A Beta Component Of Ad Amyloid Non-A4 Component Of Amyloid Precursor Nacp |
| Database Links | Reactome: R-HSA-977225 Reactome: R-HSA-9833482 |
| Cellular Localisation | Cytoplasm Membrane Nucleus Synapse Secreted Cell Projection Axon Membrane-Bound In Dopaminergic Neurons Expressed And Colocalized With Septin4 In Dopaminergic Axon Terminals Especially At The Varicosities |
| Alternative Antibody Names | Anti-Alpha-Synuclein antibody Anti-Non-A Beta Component Of Ad Amyloid antibody Anti-Non-A4 Component Of Amyloid Precursor antibody Anti-Nacp antibody Anti-SNCA antibody Anti-NACP antibody Anti-PARK1 antibody |
Information sourced from Uniprot.org