| Host: | Rabbit |
| Applications: | WB/ELISA/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Mothers against decapentaplegic homolog 3-Ser423/425 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-300 ELISA 2000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMAD3 |
| Gene ID: | 4088 |
| Uniprot ID: | SMAD3_HUMAN |
| Specificity: | This antibody detects endogenous levels of Human Mouse Rat Smad3 (phospho-Ser423 or 425) |
| Immunogen: | Synthesized phosho peptide around human Smad3 (Ser423 and 425) |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 3 Mad Homolog 3 Mad3 Mothers Against Dpp Homolog 3 Hmad-3 Jv15-2 Smad Family Member 3 Smad 3 Smad3 Hsmad3 |
| Database Links | Reactome: R-HSA-1181150 Reactome: R-HSA-1502540 Reactome: R-HSA-2173788 Reactome: R-HSA-2173789 Reactome: R-HSA-2173795 Reactome: R-HSA-2173796 Reactome: R-HSA-3304356 Reactome: R-HSA-3311021 Reactome: R-HSA-3315487 Reactome: R-HSA-3656532 Reactome: R-HSA-5689880 Reactome: R-HSA-8941855 Reactome: R-HSA-8952158 Reactome: R-HSA-9008059 Reactome: R-HSA-9013695 Reactome: R-HSA-9615017 Reactome: R-HSA-9617828 Reactome: R-HSA-9735871 Reactome: R-HSA-9754189 Reactome: R-HSA-9796292 Reactome: R-HSA-9823730 Reactome: R-HSA-9839394 |
| Cellular Localisation | Cytoplasm Nucleus Cytoplasmic And Nuclear In The Absence Of Tgf-Beta On Tgf-Beta Stimulation Migrates To The Nucleus When Complexed With Smad4 Through The Action Of The Phosphatase Ppm1a Released From The Smad2/Smad4 Complex And Exported Out Of The Nucleus By Interaction With Ranbp1 Co-Localizes With Lemd3 At The Nucleus Inner Membrane Mapk-Mediated Phosphorylation Appears To Have No Effect On Nuclear Import Pdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta Localized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm Of The Inner Cell Mass At The Blastocyst Stage |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 3 antibody Anti-Mad Homolog 3 antibody Anti-Mad3 antibody Anti-Mothers Against Dpp Homolog 3 antibody Anti-Hmad-3 antibody Anti-Jv15-2 antibody Anti-Smad Family Member 3 antibody Anti-Smad 3 antibody Anti-Smad3 antibody Anti-Hsmad3 antibody Anti-SMAD3 antibody Anti-MADH3 antibody |
Information sourced from Uniprot.org