| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S4MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPA2 |
| Gene ID: | 6118 |
| Uniprot ID: | RFA2_HUMAN |
| Specificity: | A synthetic phosphorylated peptide around T21 of human RPA2 (P15927). |
| Immunogen Sequence: | GYTQS |
| Post Translational Modifications | Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence. DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination. |
| Function | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ. |
| Protein Name | Replication Protein A 32 Kda Subunit Rp-A P32 Replication Factor A Protein 2 Rf-A Protein 2 Replication Protein A 34 Kda Subunit Rp-A P34 |
| Database Links | Reactome: R-HSA-110312 Reactome: R-HSA-110314 Reactome: R-HSA-110320 Reactome: R-HSA-174437 Reactome: R-HSA-176187 Reactome: R-HSA-3371453 Reactome: R-HSA-3371511 Reactome: R-HSA-5358565 Reactome: R-HSA-5358606 Reactome: R-HSA-5651801 Reactome: R-HSA-5655862 Reactome: R-HSA-5656121 Reactome: R-HSA-5656169 Reactome: R-HSA-5685938 Reactome: R-HSA-5685942 Reactome: R-HSA-5693607 Reactome: R-HSA-5693616 Reactome: R-HSA-5696395 Reactome: R-HSA-5696397 Reactome: R-HSA-5696400 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6783310 Reactome: R-HSA-6804756 Reactome: R-HSA-68962 Reactome: R-HSA-69166 Reactome: R-HSA-69473 Reactome: R-HSA-912446 Reactome: R-HSA-9709570 |
| Cellular Localisation | Nucleus Pml Body Redistributes To Discrete Nuclear Foci Upon Dna Damage In An Atr-Dependent Manner |
| Alternative Antibody Names | Anti-Replication Protein A 32 Kda Subunit antibody Anti-Rp-A P32 antibody Anti-Replication Factor A Protein 2 antibody Anti-Rf-A Protein 2 antibody Anti-Replication Protein A 34 Kda Subunit antibody Anti-Rp-A P34 antibody Anti-RPA2 antibody Anti-REPA2 antibody Anti-RPA32 antibody Anti-RPA34 antibody |
Information sourced from Uniprot.org