| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Replication protein A 32 kDa subunit-Ser33 (1-50 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPA2 |
| Gene ID: | 6118 |
| Uniprot ID: | RFA2_HUMAN |
| Immunogen Region: | 1-50 aa |
| Specificity: | Phospho-RPA p32 (S33) Polyclonal Antibody detects endogenous levels of RPA p32 protein only when phosphorylated at S33. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human RFA2 around the phosphorylation site of Ser33 at the amino acid range 1-50 |
| Function | As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ. |
| Protein Name | Replication Protein A 32 Kda Subunit Rp-A P32 Replication Factor A Protein 2 Rf-A Protein 2 Replication Protein A 34 Kda Subunit Rp-A P34 |
| Database Links | Reactome: R-HSA-110312 Reactome: R-HSA-110314 Reactome: R-HSA-110320 Reactome: R-HSA-174437 Reactome: R-HSA-176187 Reactome: R-HSA-3371453 Reactome: R-HSA-3371511 Reactome: R-HSA-5358565 Reactome: R-HSA-5358606 Reactome: R-HSA-5651801 Reactome: R-HSA-5655862 Reactome: R-HSA-5656121 Reactome: R-HSA-5656169 Reactome: R-HSA-5685938 Reactome: R-HSA-5685942 Reactome: R-HSA-5693607 Reactome: R-HSA-5693616 Reactome: R-HSA-5696395 Reactome: R-HSA-5696397 Reactome: R-HSA-5696400 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6783310 Reactome: R-HSA-6804756 Reactome: R-HSA-68962 Reactome: R-HSA-69166 Reactome: R-HSA-69473 Reactome: R-HSA-912446 Reactome: R-HSA-9709570 |
| Cellular Localisation | Nucleus Pml Body Redistributes To Discrete Nuclear Foci Upon Dna Damage In An Atr-Dependent Manner |
| Alternative Antibody Names | Anti-Replication Protein A 32 Kda Subunit antibody Anti-Rp-A P32 antibody Anti-Replication Factor A Protein 2 antibody Anti-Rf-A Protein 2 antibody Anti-Replication Protein A 34 Kda Subunit antibody Anti-Rp-A P34 antibody Anti-RPA2 antibody Anti-REPA2 antibody Anti-RPA32 antibody Anti-RPA34 antibody |
Information sourced from Uniprot.org