• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MKK3 (Phospho-Ser189) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from MDA-MB-435 cells, using MKK3 (Phospho-Ser189) Antibody. The lane on the left is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-MEK-3 (S218) Polyclonal Antibody

Anti-Phospho-MAP2K3-Ser218 antibody (173-222 aa) (STJ90330)

SKU:
STJ90330

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 3-Ser218 (173-222 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K3
Gene ID: 5606
Uniprot ID: MP2K3_HUMAN
Immunogen Region: 173-222 aa
Specificity: Phospho-MEK-3 (S189) Polyclonal Antibody detects endogenous levels of MEK-3 protein only when phosphorylated at S189.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MKK3 around the phosphorylation site of Ser189 at the amino acid range 173-222
Post Translational Modifications Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Function Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 3
Map Kinase Kinase 3
Mapkk 3
Mapk/Erk Kinase 3
Mek 3
Stress-Activated Protein Kinase Kinase 2
Sapk Kinase 2
Sapkk-2
Sapkk2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-450302
Reactome: R-HSA-5210891 P46734-3
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 3 antibody
Anti-Map Kinase Kinase 3 antibody
Anti-Mapkk 3 antibody
Anti-Mapk/Erk Kinase 3 antibody
Anti-Mek 3 antibody
Anti-Stress-Activated Protein Kinase Kinase 2 antibody
Anti-Sapk Kinase 2 antibody
Anti-Sapkk-2 antibody
Anti-Sapkk2 antibody
Anti-MAP2K3 antibody
Anti-MEK3 antibody
Anti-MKK3 antibody
Anti-PRKMK3 antibody
Anti-SKK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance