| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Integrin-linked protein kinase-Ser246 (212-261 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ILK |
| Gene ID: | 3611 |
| Uniprot ID: | ILK_HUMAN |
| Immunogen Region: | 212-261 aa |
| Specificity: | Phospho-ILK (S246) Polyclonal Antibody detects endogenous levels of ILK protein only when phosphorylated at S246. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human ILK around the phosphorylation site of Ser246 at the amino acid range 212-261 |
| Post Translational Modifications | Phosphorylation by PAK1 modulates ILK subcellular location by promoting its nuclear export. |
| Function | Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes. Acts as an upstream effector of both AKT1/PKB and GSK3. Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA. Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus. |
| Protein Name | Scaffold Protein Ilk Ilk-1 Ilk-2 Inactive Integrin-Linked Kinase P59ilk |
| Database Links | Reactome: R-HSA-446343 Reactome: R-HSA-446353 |
| Cellular Localisation | Cell Junction Focal Adhesion Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Projection Lamellipodium Cytoplasm Myofibril Sarcomere Nucleus Cytoskeleton Microtubule Organizing Center Centrosome Cell Cortex |
| Alternative Antibody Names | Anti-Scaffold Protein Ilk antibody Anti-Ilk-1 antibody Anti-Ilk-2 antibody Anti-Inactive Integrin-Linked Kinase antibody Anti-P59ilk antibody Anti-ILK antibody Anti-ILK1 antibody Anti-ILK2 antibody |
Information sourced from Uniprot.org