• Western blot analysis of extracts of HeLa cells, using Phospho-HSP27/HSPB1-S15 antibody (STJ22178) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5min.
  • Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g Phospho-HSP27/HSPB1-S15 polyclonal antibody (STJ22178). Western blot was performed from the immunoprecipitate using Phospho-HSP27/HSPB1-S15 polyclonal antibody (STJ22178) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight.
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-HSP27/HSPB1-S15 polyclonal antibody (STJ22178). Western blot was performed from the immunoprecipitate using Phospho-HSP27/HSPB1-S15 polyclonal antibody (STJ22178) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight.
  • Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S15 Rabbit polyclonal antibody (STJ22178) at 1:600 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-HSPB1-S15 antibody (STJ22178)

SKU:
STJ22178

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-HSPB1-S15 is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPB1
Gene ID: 3315
Uniprot ID: HSPB1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S15 of human HSP27/HSPB1 (NP_001531.1).
Immunogen Sequence: GPSWD
Tissue Specificity Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Post Translational Modifications Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.
Function Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
Protein Name Heat Shock Protein Beta-1
Hspb1
28 Kda Heat Shock Protein
Estrogen-Regulated 24 Kda Protein
Heat Shock 27 Kda Protein
Hsp 27
Stress-Responsive Protein 27
Srp27
Database Links Reactome: R-HSA-4420097
Reactome: R-HSA-450408
Reactome: R-HSA-5687128
Reactome: R-HSA-9009391
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Spindle
Cytoplasmic In Interphase Cells
Colocalizes With Mitotic Spindles In Mitotic Cells
Translocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles
Alternative Antibody Names Anti-Heat Shock Protein Beta-1 antibody
Anti-Hspb1 antibody
Anti-28 Kda Heat Shock Protein antibody
Anti-Estrogen-Regulated 24 Kda Protein antibody
Anti-Heat Shock 27 Kda Protein antibody
Anti-Hsp 27 antibody
Anti-Stress-Responsive Protein 27 antibody
Anti-Srp27 antibody
Anti-HSPB1 antibody
Anti-HSP27 antibody
Anti-HSP28 antibody

Information sourced from Uniprot.org

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