| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HSPB1 |
| Gene ID: | 3315 |
| Uniprot ID: | HSPB1_HUMAN |
| Specificity: | A synthetic phosphorylated peptide around S15 of human HSP27/HSPB1 (NP_001531.1). |
| Immunogen Sequence: | GPSWD |
| Tissue Specificity | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
| Post Translational Modifications | Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement. |
| Function | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. |
| Protein Name | Heat Shock Protein Beta-1 Hspb1 28 Kda Heat Shock Protein Estrogen-Regulated 24 Kda Protein Heat Shock 27 Kda Protein Hsp 27 Heat Shock Protein Family B Member 1 Stress-Responsive Protein 27 Srp27 |
| Database Links | Reactome: R-HSA-4420097 Reactome: R-HSA-450408 Reactome: R-HSA-5687128 Reactome: R-HSA-9009391 |
| Cellular Localisation | Cytoplasm Nucleus Cytoskeleton Spindle Cytoplasmic In Interphase Cells Colocalizes With Mitotic Spindles In Mitotic Cells Translocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles |
| Alternative Antibody Names | Anti-Heat Shock Protein Beta-1 antibody Anti-Hspb1 antibody Anti-28 Kda Heat Shock Protein antibody Anti-Estrogen-Regulated 24 Kda Protein antibody Anti-Heat Shock 27 Kda Protein antibody Anti-Hsp 27 antibody Anti-Heat Shock Protein Family B Member 1 antibody Anti-Stress-Responsive Protein 27 antibody Anti-Srp27 antibody Anti-HSPB1 antibody Anti-HSP27 antibody Anti-HSP28 antibody |
Information sourced from Uniprot.org