| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-G1/S-specific cyclin-D1-Thr286 (246-295 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CCND1 |
| Gene ID: | 595 |
| Uniprot ID: | CCND1_HUMAN |
| Immunogen Region: | 246-295 aa |
| Specificity: | Phospho-Cyclin D1 (T286) Polyclonal Antibody detects endogenous levels of Cyclin D1 protein only when phosphorylated at T286. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Cyclin D1 around the phosphorylation site of Thr286 at the amino acid range 246-295 |
| Function | Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. |
| Protein Name | G1/S-Specific Cyclin-D1 B-Cell Lymphoma 1 Protein Bcl-1 Bcl-1 Oncogene Prad1 Oncogene |
| Database Links | Reactome: R-HSA-187577 Reactome: R-HSA-1912408 Reactome: R-HSA-3214858 Reactome: R-HSA-6785807 Reactome: R-HSA-69231 Reactome: R-HSA-75815 Reactome: R-HSA-8849470 Reactome: R-HSA-8853884 Reactome: R-HSA-8878166 Reactome: R-HSA-8934593 Reactome: R-HSA-8951430 Reactome: R-HSA-8951936 Reactome: R-HSA-9018519 Reactome: R-HSA-9634638 Reactome: R-HSA-9661069 Reactome: R-HSA-9754119 Reactome: R-HSA-9825892 |
| Cellular Localisation | Nucleus Cytoplasm Nucleus Membrane Cyclin D-Cdk4 Complexes Accumulate At The Nuclear Membrane And Are Then Translocated To The Nucleus Through Interaction With Kip/Cip Family Members |
| Alternative Antibody Names | Anti-G1/S-Specific Cyclin-D1 antibody Anti-B-Cell Lymphoma 1 Protein antibody Anti-Bcl-1 antibody Anti-Bcl-1 Oncogene antibody Anti-Prad1 Oncogene antibody Anti-CCND1 antibody Anti-BCL1 antibody Anti-PRAD1 antibody |
Information sourced from Uniprot.org