| Host: | Rabbit |
| Applications: | WB/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Caspase-9-Tyr153 (119-168 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IF 1:200-1:1000 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CASP9 |
| Gene ID: | 842 |
| Uniprot ID: | CASP9_HUMAN |
| Immunogen Region: | 119-168 aa |
| Specificity: | Phospho-Caspase-9 (Y153) Polyclonal Antibody detects endogenous levels of Caspase-9 protein only when phosphorylated at Y153. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Caspase 9 around the phosphorylation site of Tyr153 at the amino acid range 119-168 |
| Post Translational Modifications | Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl.occurs in the response of cells to DNA damage. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP9 processing, preventing CASP9 activation and ability to mediate intrinsic apoptosis. Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC. Isoform 2: Lacks activity is an dominant-negative inhibitor of caspase-9. |
| Protein Name | Caspase-9 Casp-9 Apoptotic Protease Mch-6 Apoptotic Protease-Activating Factor 3 Apaf-3 Ice-Like Apoptotic Protease 6 Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 |
| Database Links | Reactome: R-HSA-111458 Reactome: R-HSA-111459 Reactome: R-HSA-111463 Reactome: R-HSA-111464 Reactome: R-HSA-168638 Reactome: R-HSA-198323 Reactome: R-HSA-418889 Reactome: R-HSA-5674400 Reactome: R-HSA-9627069 |
| Alternative Antibody Names | Anti-Caspase-9 antibody Anti-Casp-9 antibody Anti-Apoptotic Protease Mch-6 antibody Anti-Apoptotic Protease-Activating Factor 3 antibody Anti-Apaf-3 antibody Anti-Ice-Like Apoptotic Protease 6 antibody Anti-Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 antibody Anti-CASP9 antibody Anti-MCH6 antibody |
Information sourced from Uniprot.org