• Western blot analysis of extracts of various cell lines, using PHKA2 antibody (STJ111771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PHKA2 antibody (620-780) (STJ111771)

SKU:
STJ111771

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHKA2 (620-780) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHKA2
Gene ID: 5256
Uniprot ID: KPB2_HUMAN
Immunogen Region: 620-780
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 620-780 of human PHKA2 (NP_000283.1).
Immunogen Sequence: DPDCDEKLFDNASEGTFSPD SDSDLVGYLEDTCNQESQDE LDHYINHLLQSTSLRSYLPP LCKNTEDRHVFSAIHSTRDI LSVMAKAKGLEVPFVPMTLP TKVLSAHRKSLNLVDSPQPL LEKVPESDFQWPRDDHGDVD CEKLVEQLKDCSNLQDQADI L
Tissue Specificity Predominantly expressed in liver and other non-muscle tissues.
Post Translational Modifications Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
Function Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Protein Name Phosphorylase B Kinase Regulatory Subunit Alpha - Liver Isoform
Phosphorylase Kinase Alpha L Subunit
Database Links Reactome: R-HSA-70221
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Phosphorylase B Kinase Regulatory Subunit Alpha - Liver Isoform antibody
Anti-Phosphorylase Kinase Alpha L Subunit antibody
Anti-PHKA2 antibody
Anti-PHKLA antibody
Anti-PYK antibody

Information sourced from Uniprot.org

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