Western blot analysis of various lysates using PFN4 Rabbit polyclonal antibody (STJ115647) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-PFN4 antibody (1-129) (STJ115647)

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STJ115647
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFN4
Gene ID: 375189
Uniprot ID: PROF4_HUMAN
Immunogen Region: 1-129
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-129 of human PFN4 (NP_955378.1).
Immunogen Sequence: MSHLQSLLLDTLLGTKHVDS AALIKIQERSLCVASPGFNV TPSDVRTLVNGFAKNPLQAR REGLYFKGKDYRCVRADEYS LYAKNENTGVVVVKTHLYLL VATYTEGMYPSICVEATESL GDYLRKKGS
Tissue Specificity Expressed in testis, in germ cells in seminiferous tubules (at protein level). Prominently expressed in pachytene/diplotene stage spematocytes.
Function Involved in male fertility. Required for manchette development and acrosome biogenesis during spermiogenesis. Binds in vitro to phospholipids, including phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidic acid (PA). Contrary to other profilin family members, does not bind to actin in vitro.
Protein Name Profilin-4
Profilin Iv
Cellular Localisation Cytoplasm
In Round Spermatids
Mainly Observed In The Acroplaxome
During The Progression Of Spermiogenesis
Relocalizes To The Developing Manchette Of Spermatids Step 8 (S8)
Coinciding With The Initiation Of Manchette Disassembly In Spermatids S14
Seen In The Cytoplasm Subjacent To The Disassembling Manchette
Alternative Antibody Names Anti-Profilin-4 antibody
Anti-Profilin Iv antibody
Anti-PFN4 antibody

Information sourced from Uniprot.org

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