| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PEX5 (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PEX5 |
| Gene ID: |
5830 |
| Uniprot ID: |
PEX5_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PEX5 (NP_000310.2). |
| Immunogen Sequence: |
MAMRELVEAECGGANPLMKL AGHFTQDKALRQEGLRPGPW PPGAPASEAASKPLGVASED ELVAEFLQDQNAPLVSRAPQ TFKMDDLLAEMQQIEQSNFR QAPQRAPGVADLALSENWAQ EFLAAGDAVDVTQDYNETDW SQEFISEVTDPLSVSPARWA EEYLEQSEEKLWLGEPEGTA TDRWYDEYHPEEDLQHTASD FVAKVDDPKLANSEFLKFVR QIGEGQVSLESGAGSGRAQ |
| Tissue Specificity | Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Monoubiquitinated at Cys-11 by PEX2 during PEX5 passage through the retrotranslocation channel. Cys-11 monoubiquitination acts as a recognition signal for the PEX1-PEX6 complex and is required for PEX5 extraction and export from peroxisomes. Monoubiquitination at Cys-11 is removed by USP9X in the cytosol, resetting PEX5 for a subsequent import cycle. When PEX5 recycling is compromised, polyubiquitinated by PEX10 during its passage through the retrotranslocation channel, leading to its degradation. Monoubiquitination at Lys-472 by TRIM37 promotes its stability by preventing its polyubiquitination and degradation by the proteasome. Ubiquitination at Lys-527 is not mediated by the PEX2-PEX10-PEX12 ligase complex and is not related to PEX5 recycling. Monoubiquitinated at Lys-209 by PEX2 following phosphorylation by ATM in response to starvation or reactive oxygen species (ROS), leading to PEX5 recognition by p62/SQSTM1 and induction of pexophagy. Phosphorylated at Ser-141 by ATM in response to reactive oxygen species (ROS), promoting monoubiquitination at Lys-209 and induction of pexophagy. |
| Function | Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle. Isoform 1: In addition to promoting peroxisomal translocation of proteins containing a PTS1 peroxisomal targeting signal, mediates peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal via its interaction with PEX7. Interaction with PEX7 only takes place when PEX7 is associated with cargo proteins containing a PTS2 peroxisomal targeting signal. PEX7 along with PTS2-containing cargo proteins are then translocated through the PEX13-PEX14 docking complex together with PEX5. Isoform 2: Does not mediate translocation of peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal. |
| Protein Name | Peroxisomal Targeting Signal 1 ReceptorPts1 ReceptorPts1rPts1-BpPeroxin-5Peroxisomal C-Terminal Targeting Signal Import ReceptorPeroxisome Receptor 1 |
| Database Links | Reactome: R-HSA-8866654Reactome: R-HSA-9033241Reactome: R-HSA-9664873 |
| Cellular Localisation | CytoplasmCytosolPeroxisome MatrixCycles Between The Cytosol And The Peroxisome MatrixFollowing Binding To Cargo Proteins Containing A Pts1 Peroxisomal Targeting Signal In The CytosolRecruited To The Docking ComplexComposed Of Pex13 And Pex14Leading To Translocation Into The Peroxisome Matrix Along With Cargo ProteinsExport And Recycling To The Cytosol Is Initiated By Binding To The Pex2-Pex10-Pex12 Ligase Complex Via Its Unstructured N-Terminus That Inserts Into The Ligase Pore And Emerges In The CytosolCys-11 Of Pex5 Is Then MonoubiquitinatedPromoting Its Extraction From Peroxisomal Membrane By The Pex1-Pex6 Aaa Atpase ComplexExtraction Is Accompanied By Unfolding Of The Tpr Repeats And Release Of Bound Cargo In The Peroxisome MatrixThe Tpr Repeats Refold In The Cytosol And Ubiquitination Is Removed By Deubiquitinating EnzymesResetting Pex5 For A Subsequent Import Cycle |
| Alternative Antibody Names | Anti-Peroxisomal Targeting Signal 1 Receptor antibodyAnti-Pts1 Receptor antibodyAnti-Pts1r antibodyAnti-Pts1-Bp antibodyAnti-Peroxin-5 antibodyAnti-Peroxisomal C-Terminal Targeting Signal Import Receptor antibodyAnti-Peroxisome Receptor 1 antibodyAnti-PEX5 antibodyAnti-PXR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
