• Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of paraffin-embedded rat liver using perilipin-2 Rabbit polyclonal antibody (STJ11105333) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of perilipin-2 in paraffin-embedded rat liver tissue using perilipin-2 Rabbit polyclonal antibody (STJ11105333) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-perilipin-2 antibody (325-425) (STJ11105333)

SKU:
STJ11105333

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-perilipin-2 (325-425) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Plin2
Gene ID: 11520
Uniprot ID: PLIN2_MOUSE
Immunogen Region: 325-425
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 325-425 of mouse perilipin-2 (NP_031434.3).
Immunogen Sequence: TVLVNAQGLPQNIQDQAKHL GVMAGDIYSVFRNAASFKEV SDGVLTSSKGQLQKMKESLD EVMDYFVNNTPLNWLVGPFY PQSTEVNKASLKVQQSEVKA Q
Tissue Specificity Adipose tissue specific. Expressed abundantly and preferentially in fat pads.
Post Translational Modifications Acylated.primarily with C14, C16 and C18 fatty acids. Phosphorylation at Tyr-230 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.
Function Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Protein Name Perilipin-2
Adipophilin
Adipose Differentiation-Related Protein
Adrp
Cellular Localisation Membrane
Peripheral Membrane Protein
Lipid Droplet
Alternative Antibody Names Anti-Perilipin-2 antibody
Anti-Adipophilin antibody
Anti-Adipose Differentiation-Related Protein antibody
Anti-Adrp antibody
Anti-Plin2 antibody
Anti-Adfp antibody
Anti-Adrp antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance