| Host: | Rat |
| Applications: | ICFC |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description : | Rat monoclonal antibody anti-Perforin-1 is suitable for use in IConfocal MicroscopyC research applications. |
| Clonality : | Monoclonal |
| Clone ID : | S16009A |
| Conjugation: | AF488 |
| Isotype: | IgG2ak |
| Formulation: | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
| Concentration: | 5 Mu L/Test |
| Storage Instruction: | Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
| Gene Symbol: | Prf1 |
| Gene ID: | 18646 |
| Uniprot ID: | PERF_MOUSE |
| Tissue Specificity | Detected in cytotoxic T-lymphocytes and natural killer cells. |
| Post Translational Modifications | N-glycosylated. The glycosylation sites are facing the interior of the pore. |
| Function | Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu. |
| Protein Name | Perforin-1 P1 Cytolysin Lymphocyte Pore-Forming Protein |
| Cellular Localisation | Cytolytic Granule Secreted Cell Membrane Multi-Pass Membrane Protein Endosome Lumen Released From Cytotoxic Lymphocytes Together With Proapoptotic Granzymes: Stored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes Inserts Into The Cell Membrane Of Target Cells And Forms Pores Membrane Insertion And Pore Formation Requires A Major Conformation Change |
| Alternative Antibody Names | Anti-Perforin-1 antibody Anti-P1 antibody Anti-Cytolysin antibody Anti-Lymphocyte Pore-Forming Protein antibody Anti-Prf1 antibody Anti-Pfp antibody |
Information sourced from Uniprot.org