| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDGFRA |
| Gene ID: | 5156 |
| Uniprot ID: | PGFRA_HUMAN |
| Immunogen Region: | 896-1089 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 896-1059 of human PDGFR alpha (NP_006197.1). |
| Immunogen Sequence: | YPGMMVDSTFYNKIKSGYRM AKPDHATSEVYEIMVKCWNS EPEKRPSFYHLSEIVENLLP GQYKKSYEKIHLDFLKSDHP AVARMRVDSDNAYIGVTYKN EEDKLKDWEGGLDEQRLSAD SGYIIPLPDIDPVPEEEDLG KRNRHSSQTSEESAIETGSS SSTF |
| Tissue Specificity | Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue. |
| Post Translational Modifications | N-glycosylated. Ubiquitinated, leading to its internalization and degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-731 and Tyr-742 is important for interaction with PIK3R1. Phosphorylation at Tyr-720 and Tyr-754 is important for interaction with PTPN11. Phosphorylation at Tyr-762 is important for interaction with CRK. Phosphorylation at Tyr-572 and Tyr-574 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-988 and Tyr-1018 is important for interaction with PLCG1. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades.the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor. |
| Protein Name | Platelet-Derived Growth Factor Receptor Alpha Pdgf-R-Alpha Pdgfr-Alpha Alpha Platelet-Derived Growth Factor Receptor Alpha-Type Platelet-Derived Growth Factor Receptor Cd140 Antigen-Like Family Member A Cd140a Antigen Platelet-Derived Growth Factor Alpha Receptor Platelet-Derived Growth Factor Receptor 2 Pdgfr-2 Cd Antigen Cd140a |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-186763 Reactome: R-HSA-186797 Reactome: R-HSA-2219530 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9674396 Reactome: R-HSA-9674401 Reactome: R-HSA-9674403 Reactome: R-HSA-9674404 Reactome: R-HSA-9674428 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Cilium Golgi Apparatus |
| Alternative Antibody Names | Anti-Platelet-Derived Growth Factor Receptor Alpha antibody Anti-Pdgf-R-Alpha antibody Anti-Pdgfr-Alpha antibody Anti-Alpha Platelet-Derived Growth Factor Receptor antibody Anti-Alpha-Type Platelet-Derived Growth Factor Receptor antibody Anti-Cd140 Antigen-Like Family Member A antibody Anti-Cd140a Antigen antibody Anti-Platelet-Derived Growth Factor Alpha Receptor antibody Anti-Platelet-Derived Growth Factor Receptor 2 antibody Anti-Pdgfr-2 antibody Anti-Cd Antigen Cd140a antibody Anti-PDGFRA antibody Anti-PDGFR2 antibody Anti-RHEPDGFRA antibody |
Information sourced from Uniprot.org