| Host: | Rabbit |
| Applications: | IHC/IF/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Poly polymerase 1 ADP-ribosyltransferase 1 polymerase 1, processed C-terminus; Poly polymerase 1, processed N-terminus (410-460 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot resea |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC-P 1:50-200 WB 1:500-2000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PARP1 |
| Gene ID: | 142 |
| Uniprot ID: | PARP1_HUMAN |
| Immunogen Region: | 410-460 aa |
| Specificity: | This antibody detects endogenous levels of human PARP-1 |
| Immunogen: | Synthesized peptide derived from the human PARP-1 at the amino acid range 410-460 |
| Function | Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine, histidine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Specificity for the different amino acids is conferred by interacting factors, such as HPF1 and NMNAT1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins.HPF1 confers serine specificity by completing the PARP1 active site. Also catalyzes tyrosine ADP-ribosylation of target proteins following interaction with HPF1. Following interaction with NMNAT1, catalyzes glutamate and aspartate ADP-ribosylation of target proteins.NMNAT1 confers glutamate and aspartate specificity. PARP1 initiates the repair of DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones (H2BS6ADPr and H3S10ADPr), thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks. HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP1 in order to limit the length of poly-ADP-ribose chains. In addition to base excision repair (BER) pathway, also involved in double-strand breaks (DSBs) repair: together with TIMELESS, accumulates at DNA damage sites and promotes homologous recombination repair by mediating poly-ADP-ribosylation. Mediates the poly-ADP-ribosylation of a number of proteins, including itself, APLF, CHFR, RPA1 and NFAT5. In addition to proteins, also able to ADP-ribosylate DNA: catalyzes ADP-ribosylation of DNA strand break termini containing terminal phosphates and a 2'-OH group in single- and double-stranded DNA, respectively. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. PARP1-mediated DNA repair in neurons plays a role in sleep: senses DNA damage in neurons and promotes sleep, facilitating efficient DNA repair. In addition to DNA repair, also involved in other processes, such as transcription regulation, programmed cell death, membrane repair, adipogenesis and innate immunity. Acts as a repressor of transcription: binds to nucleosomes and modulates chromatin structure in a manner similar to histone H1, thereby altering RNA polymerase II. Acts both as a positive and negative regulator of transcription elongation, depending on the context. Acts as a positive regulator of transcription elongation by mediating poly-ADP-ribosylation of NELFE, preventing RNA-binding activity of NELFE and relieving transcription pausing. Acts as a negative regulator of transcription elongation in response to DNA damage by catalyzing poly-ADP-ribosylation of CCNT1, disrupting the phase separation activity of CCNT1 and subsequent activation of CDK9. Involved in replication fork progression following interaction with CARM1: mediates poly-ADP-ribosylation at replication forks, slowing fork progression. Poly-ADP-ribose chains generated by PARP1 also play a role in poly-ADP-ribose-dependent cell death, a process named parthanatos. Also acts as a negative regulator of the cGAS-STING pathway. Acts by mediating poly-ADP-ribosylation of CGAS: PARP1 translocates into the cytosol following phosphorylation by PRKDC and catalyzes poly-ADP-ribosylation and inactivation of CGAS. Acts as a negative regulator of adipogenesis: catalyzes poly-ADP-ribosylation of histone H2B on 'Glu-35' (H2BE35ADPr) following interaction with NMNAT1, inhibiting phosphorylation of H2B at 'Ser-36' (H2BS36ph), thereby blocking expression of pro-adipogenetic genes. Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Poly ADP-ribose polymerase 1, processed C-terminus: Promotes AIFM1-mediated apoptosis. This form, which translocates into the cytoplasm following cleavage by caspase-3 (CASP3) and caspase-7 (CASP7) in response to apoptosis, is auto-poly-ADP-ribosylated and serves as a poly-ADP-ribose carrier to induce AIFM1-mediated apoptosis. Poly ADP-ribose polymerase 1, processed N-terminus: This cleavage form irreversibly binds to DNA breaks and interferes with DNA repair, promoting DNA damage-induced apoptosis. |
| Protein Name | Poly Adp-Ribose Polymerase 1 Parp-1 Adp-Ribosyltransferase Diphtheria Toxin-Like 1 Artd1 Dna Adp-Ribosyltransferase Parp1 Nad(+ Adp-Ribosyltransferase 1 Adprt 1 PolyAdp-Ribose Synthase 1 Protein Poly-Adp-Ribosyltransferase Parp1 Cleaved Into - Poly Adp-Ribose Polymerase 1 - Processed C-Terminus Poly Adp-Ribose Polymerase 1 - 89-Kda Form - Poly Adp-Ribose Polymerase 1 - Processed N-Terminus Nt-Parp-1 Poly Adp-Ribose Polymerase 1 - 24-Kda Form Poly Adp-Ribose Polymerase 1 - 28-Kda Form |
| Database Links | Reactome: R-HSA-110362 Reactome: R-HSA-192814 Reactome: R-HSA-2173795 Reactome: R-HSA-3108214 Reactome: R-HSA-5685939 Reactome: R-HSA-5696394 Reactome: R-HSA-5696395 Reactome: R-HSA-5696400 |
| Cellular Localisation | Chromosome Nucleus Nucleolus Cytoplasm Cytosol Localizes To Sites Of Dna Damage Recognizes (Via Parp-Type Zinc-Fingers) And Binds Dna Strand Breaks Also Binds Normal/Undamaged Chromatin Auto Poly-Adp-Ribosylation Promotes Dissociation From Chromatin Extracted From Chromatin By Vcp/P97 Following Sumoylation And Ubiquitination Translocates From The Nucleus To The Cytosol Following Phosphorylation By Prkdc Recruited To Replication Forks Following Interaction With Carm1 Poly Adp-Ribose Polymerase 1 Processed N-Terminus: Chromosome Following Cleavage By Caspase-3 (Casp3) And Caspase-7 (Casp7) In Response To Apoptosis This Cleavage Form Irreversibly Binds To Dna Breaks Processed C-Terminus: Cytoplasm Translocates Into The Cytoplasm Where The Auto-Poly-Adp-Ribosylated Form Serves As A Poly-Adp-Ribose Carrier To Induce Aifm1-Mediated Apoptosis |
| Alternative Antibody Names | Anti-Poly Adp-Ribose Polymerase 1 antibody Anti-Parp-1 antibody Anti-Adp-Ribosyltransferase Diphtheria Toxin-Like 1 antibody Anti-Artd1 antibody Anti-Dna Adp-Ribosyltransferase Parp1 antibody Anti-Nad(+ Adp-Ribosyltransferase 1 antibody Anti-Adprt 1 antibody Anti-PolyAdp-Ribose Synthase 1 antibody Anti-Protein Poly-Adp-Ribosyltransferase Parp1 Cleaved Into - Poly Adp-Ribose Polymerase 1 - Processed C-Terminus antibody Anti-Poly Adp-Ribose Polymerase 1 - 89-Kda Form - Poly Adp-Ribose Polymerase 1 - Processed N-Terminus antibody Anti-Nt-Parp-1 antibody Anti-Poly Adp-Ribose Polymerase 1 - 24-Kda Form antibody Anti-Poly Adp-Ribose Polymerase 1 - 28-Kda Form antibody Anti-PARP1 antibody Anti-ADPRT antibody Anti-PPOL antibody |
Information sourced from Uniprot.org