• Immunofluorescence analysis of MCF7 cells using Pannexin 1 antibody (STJ115548). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Pannexin 1 antibody (STJ115548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PANX1 antibody (297-426) (STJ115548)

SKU:
STJ115548

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PANX1 (297-426) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PANX1
Gene ID: 24145
Uniprot ID: PANX1_HUMAN
Immunogen Region: 297-426
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 297-426 of human Pannexin 1 (NP_056183.2).
Immunogen Sequence: VPFRQKTDVLKVYEILPTFD VLHFKSEGYNDLSLYNLFLE ENISEVKSYKCLKVLENIKS SGQGIDPMLLLTNLGMIKMD VVDGKTPMSAEMREEQGNQT AELQGMNIDSETKANNGEKN ARQRLLDSSC
Tissue Specificity Widely expressed. Highest expression is observed in oocytes and brain. Detected at very low levels in sperm cells.
Post Translational Modifications S-nitrosylation inhibits channel currents and ATP release. N-glycosylation may play a role in cell surface targeting. Exists in three glycosylation states: non-glycosylated (GLY0), high-mannose glycosylated (GLY1), and fully mature glycosylated (GLY2).
Function Structural component of the gap junctions and the hemichannels involved in the ATP release and nucleotide permeation. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis. Plays a critical role in oogenesis.
Protein Name Pannexin-1
Database Links Reactome: R-HSA-112303
Reactome: R-HSA-844456
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Pannexin-1 antibody
Anti-PANX1 antibody
Anti-MRS1 antibody
Anti-UNQ2529 antibody
Anti-PRO6028 antibody

Information sourced from Uniprot.org

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