• Western blot analysis of extracts of various cell lines, using PAFAH1B2 antibody (STJ114063) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PAFAH1B2 antibody (1-229) (STJ114063)

SKU:
STJ114063

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PAFAH1B2 (1-229) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PAFAH1B2
Gene ID: 5049
Uniprot ID: PA1B2_HUMAN
Immunogen Region: 1-229
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human PAFAH1B2 (NP_002563.1).
Immunogen Sequence: MSQGDSNPAAIPHAAEDIQG DDRWMSQHNRFVLDCKDKEP DVLFVGDSMVQLMQQYEIWR ELFSPLHALNFGIGGDTTRH VLWRLKNGELENIKPKVIVV WVGTNNHENTAEEVAGGIEA IVQLINTRQPQAKIIVLGLL PRGEKPNPLRQKNAKVNQLL KVSLPKLANVQLLDTDGGFV HSDGAISCHDMFDFLHLTGG GYAKICKPLHELIMQLLEET PEEKQTTIA
Tissue Specificity Ubiquitous.
Function Alpha2 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. The alpha2/alpha2 homodimer (PAFAH1B2/PAFAH1B2 homodimer) hydrolyzes PAF and 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE) more efficiently than 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA). In contrast, the alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B3 heterodimer) hydrolyzes AAGPA more efficiently than PAF, but has little hydrolytic activity towards AAGPE. May play a role in male germ cell meiosis during the late pachytenestage and meiotic divisions as well as early spermiogenesis.
Protein Name Platelet-Activating Factor Acetylhydrolase Ib Subunit Alpha2
Paf Acetylhydrolase 30 Kda Subunit
Paf-Ah 30 Kda Subunit
Paf-Ah Subunit Beta
Pafah Subunit Beta
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6811436
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Platelet-Activating Factor Acetylhydrolase Ib Subunit Alpha2 antibody
Anti-Paf Acetylhydrolase 30 Kda Subunit antibody
Anti-Paf-Ah 30 Kda Subunit antibody
Anti-Paf-Ah Subunit Beta antibody
Anti-Pafah Subunit Beta antibody
Anti-PAFAH1B2 antibody
Anti-PAFAHB antibody

Information sourced from Uniprot.org

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