| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-NTHL1 (1-100) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.64-0.72 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | NTHL1 |
| Gene ID: | 4913 |
| Uniprot ID: | NTH_HUMAN |
| Immunogen Region: | 1-100 |
| Immunogen: | N-epitope synthetic peptide taken within amino acid region 1-100 on human NTHL1 protein. |
| Tissue Specificity | Widely expressed with highest levels in heart and lowest levels in lung and liver. |
| Post Translational Modifications | Ubiquitinated by TRIM26.leading to proteasomal degradation. |
| Function | Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption. |
| Protein Name | Endonuclease Iii-Like Protein 1 Hnth1 Bifunctional Dna N-Glycosylase/Dna-(Apurinic Or Apyrimidinic Site Lyase Dna Glycosylase/Ap Lyase |
| Database Links | Reactome: R-HSA-110328 Reactome: R-HSA-110329 Reactome: R-HSA-110357 Reactome: R-HSA-9630221 Reactome: R-HSA-9630222 |
| Cellular Localisation | Nucleus Mitochondrion |
| Alternative Antibody Names | Anti-Endonuclease Iii-Like Protein 1 antibody Anti-Hnth1 antibody Anti-Bifunctional Dna N-Glycosylase/Dna-(Apurinic Or Apyrimidinic Site Lyase antibody Anti-Dna Glycosylase/Ap Lyase antibody Anti-NTHL1 antibody Anti-NTH1 antibody Anti-OCTS3 antibody |
Information sourced from Uniprot.org