• Immunofluorescence analysis of MCF-7 cells using NSUN6 antibody (STJ115415). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using NSUN6 antibody (STJ115415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NSUN6 antibody (220-469) (STJ115415)

SKU:
STJ115415

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NSUN6 (220-469) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSUN6
Gene ID: 221078
Uniprot ID: NSUN6_HUMAN
Immunogen Region: 220-469
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1).
Immunogen Sequence: NLPSALVSHVLNPQPGEKIL DLCAAPGGKTTHIAALMHDQ GEVIALDKIFNKVEKIKQNA LLLGLNSIRAFCFDGTKAVK LDMVEDTEGEPPFLPESFDR ILLDAPCSGMGQRPNMACTW SVKEVASYQPLQRKLFTAAV QLLKPEGVLVYSTCTITLAE NEEQVAWALTKFPCLQLQPQ EPQIGGEGMRGAGLSCEQLK QLQRFDPSAVPLPDTDMDSL REARREDMLRLANKDSIGF
Function S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.
Protein Name Trna
Cytosine(72-C(5-Methyltransferase Nsun6
Nol1/Nop2/Sun And Pua Domain-Containing Protein 1
Nol1/Nop2/Sun Domain Family Member 6
Database Links Reactome: R-HSA-6782315
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Trna antibody
Anti-Cytosine(72-C(5-Methyltransferase Nsun6 antibody
Anti-Nol1/Nop2/Sun And Pua Domain-Containing Protein 1 antibody
Anti-Nol1/Nop2/Sun Domain Family Member 6 antibody
Anti-NSUN6 antibody
Anti-NOPD1 antibody

Information sourced from Uniprot.org

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