| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Nop30 (C-Term) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 0.02% NaN3 |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:500-1:1000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NOL3 |
| Gene ID: | 8996 |
| Uniprot ID: | NOL3_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | Reacts with the C-terminal region of the 25 kDa human Nop30 protein. Since ARC and Nop30 have different C-termini, this antibody is specific for Nop30. |
| Immunogen: | Synthetic peptide corresponding to amino acids from the C-terminal region of the human Nop30 protein |
| Post Translational Modifications | Phosphorylation at Thr-149 is required for its antiapoptotic effect by blocking death-inducing signaling complex death-inducing signaling complex (DISC) activity through the control of interaction with CASP8. Phosphorylation at Thr-149 results in translocation to mitochondria and this translocation enables the binding to CASP8. Dephosphorylated at Thr-149 by calcineurin.doesn't inhibit the association between FADD and CASP8 and the consequent apoptosis. Polyubiquitinated by MDM2.promoting proteasomal-dependent degradation in response to apoptotic stimuli. |
| Function | Isoform 1: May be involved in RNA splicing. Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation. Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors. Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis. Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation. Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner. Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I. Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia. Inhibits too myoblast differentiation through caspase inhibition. |
| Protein Name | Nucleolar Protein 3 Apoptosis Repressor With Card Muscle-Enriched Cytoplasmic Protein Myp Nucleolar Protein Of 30 Kda Nop30 |
| Cellular Localisation | Isoform 1: Nucleus Nucleolus The Sr-Rich C-Terminus Mediates Nuclear Localization Isoform 3: Cytoplasm Isoform 2: Cytoplasm Mitochondrion Sarcoplasmic Reticulum Membrane Lipid-Anchor Phosphorylation At Thr-149 Results In Translocation To Mitochondria Colocalized With Mitochondria In Response To Oxidative Stress |
| Alternative Antibody Names | Anti-Nucleolar Protein 3 antibody Anti-Apoptosis Repressor With Card antibody Anti-Muscle-Enriched Cytoplasmic Protein antibody Anti-Myp antibody Anti-Nucleolar Protein Of 30 Kda antibody Anti-Nop30 antibody Anti-NOL3 antibody Anti-ARC antibody Anti-NOP antibody |
Information sourced from Uniprot.org