• Immunohistochemistry analysis of paraffin-embedded human stomach using NME3 antibody (STJ24774) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NME3 antibody (STJ24774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NME3 antibody (20-169) (STJ24774)

SKU:
STJ24774

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NME3 (20-169) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NME3
Gene ID: 4832
Uniprot ID: NDK3_HUMAN
Immunogen Region: 20-169
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-169 of human NME3 (NP_002504.2).
Immunogen Sequence: AHERTFLAVKPDGVQRRLVG EIVRRFERKGFKLVALKLVQ ASEELLREHYAELRERPFYG RLVKYMASGPVVAMVWQGLD VVRTSRALIGATNPADAPPG TIRGDFCIEVGKNLIHGSDS VESARREIALWFRADELLCW EDSAGHWLYE
Function Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis.
Protein Name Nucleoside Diphosphate Kinase 3
Ndk 3
Ndp Kinase 3
Dr-Nm23
Nucleoside Diphosphate Kinase C
Ndpkc
Nm23-H3
Database Links Reactome: R-HSA-499943
Alternative Antibody Names Anti-Nucleoside Diphosphate Kinase 3 antibody
Anti-Ndk 3 antibody
Anti-Ndp Kinase 3 antibody
Anti-Dr-Nm23 antibody
Anti-Nucleoside Diphosphate Kinase C antibody
Anti-Ndpkc antibody
Anti-Nm23-H3 antibody
Anti-NME3 antibody

Information sourced from Uniprot.org

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