• Immunofluorescence analysis of PC-12 cells using NKIRAS1 Rabbit polyclonal antibody (STJ117078) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using NKIRAS1 Rabbit polyclonal antibody (STJ117078) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NKIRAS1 Rabbit polyclonal antibody (STJ117078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NKIRAS1 antibody (118-192) (STJ117078)

SKU:
STJ117078

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NKIRAS1 (118-192) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NKIRAS1
Gene ID: 28512
Uniprot ID: KBRS1_HUMAN
Immunogen Region: 118-192
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 118-192 of human NKIRAS1 (NP_065078.1).
Immunogen Sequence: LGNKIDLSEQRQVDAEVAQQ WAKSEKVRLWEVTVTDRKTL IEPFTLLASKLSQPQSKSSF PLPGRKNKGNSNSEN
Tissue Specificity Widely expressed.
Function Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
Protein Name Nf-Kappa-B Inhibitor-Interacting Ras-Like Protein 1
I-Kappa-B-Interacting Ras-Like Protein 1
Kappa B-Ras Protein 1
Kappab-Ras1
Database Links Reactome: R-HSA-1810476
Reactome: R-HSA-445989
Reactome: R-HSA-933542
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor-Interacting Ras-Like Protein 1 antibody
Anti-I-Kappa-B-Interacting Ras-Like Protein 1 antibody
Anti-Kappa B-Ras Protein 1 antibody
Anti-Kappab-Ras1 antibody
Anti-NKIRAS1 antibody
Anti-KBRAS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance