| Host: | Mouse |
| Applications: | IHC/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Nuclear factor of activated T-cells 5 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 1D6 |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC-P 1:50-200 WB 1:1000-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NFAT5 |
| Gene ID: | 10725 |
| Uniprot ID: | NFAT5_HUMAN |
| Specificity: | This antibody detects endogenous levels of NFAT5 at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from the human NFAT5 |
| Post Translational Modifications | Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress.this phosphorylation mediates its rapid nuclear localization. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage. |
| Function | Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-ACTAGTGGAAACATATAATCCAATGGTGACCGCT-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes.optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops.R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. |
| Protein Name | Nuclear Factor Of Activated T-Cells 5 Nf-At5 T-Cell Transcription Factor Nfat5 Tonicity-Responsive Enhancer-Binding Protein Tone-Binding Protein Tonebp |
| Cellular Localisation | Nucleus Cytoplasm Chromosome Nuclear Distribution Increases Under Hypertonic Conditions Recruited To Sites Of R-Loop-Associated Dna Damage Following Poly-Adp-Ribosylation By Parp1 |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells 5 antibody Anti-Nf-At5 antibody Anti-T-Cell Transcription Factor Nfat5 antibody Anti-Tonicity-Responsive Enhancer-Binding Protein antibody Anti-Tone-Binding Protein antibody Anti-Tonebp antibody Anti-NFAT5 antibody Anti-KIAA0827 antibody Anti-TONEBP antibody |
Information sourced from Uniprot.org