• STJ71114 (2µg/ml) staining of Human Pancrease lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71114 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing mitochondria/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71114 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Neuroligin 2 antibody (Internal) (STJ71114)

SKU:
STJ71114-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Neuroligin 2 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-2-3µg/ml
IF-Strong expression of the protein seen in the mitochondria/cytoplasm of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NLGN2
Gene ID: 57555
Uniprot ID: NLGN2_HUMAN
Immunogen Region: Internal
Accession Number: NP_065846.1
Immunogen Sequence: NPPDTDIRDPGKK
Function Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Plays a role in synapse function and synaptic signal transmission, especially via gamma-aminobutyric acid receptors (GABA(A) receptors). Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Promotes clustering of postsynaptic LHFPL4. Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion.
Protein Name Neuroligin-2
Database Links Reactome: R-HSA-6794361
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Postsynaptic Cell Membrane
Presynaptic Cell Membrane
Detected At Postsynaptic Membranes In Brain
Detected At Dendritic Spines In Cultured Neurons
Colocalizes With Gphn And Arhgef9 At Neuronal Cell Membranes
Localized At Presynaptic Membranes In Retina
Colocalizes With Gabrg2 At Inhibitory Synapses In The Retina
Alternative Antibody Names Anti-Neuroligin-2 antibody
Anti-NLGN2 antibody
Anti-KIAA1366 antibody

Information sourced from Uniprot.org

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