• Western blot analysis of extracts of various cell lines, using NDUFAF5 antibody (STJ29215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NDUFAF5 antibody (196-345) (STJ29215)

SKU:
STJ29215

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFAF5
Gene ID: 79133
Uniprot ID: NDUF5_HUMAN
Immunogen Region: 196-345
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 196-345 of human NDUFAF5 (NP_077025.2).
Immunogen Sequence: ELRCSLQLAETEREGGFSPH ISPFTAVNDLGHLLGRAGFN TLTVDTDEIQVNYPGMFELM EDLQGMGESNCAWNRKALLH RDTMLAAAAVYREMYRNEDG SVPATYQIYYMIGWKYHESQ ARPAERGSATVSFGELGKIN NLMPPGKKSQ
Function Arginine hydroxylase involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. Acts by mediating hydroxylation of 'Arg-111' of NDUFS7. May also have methyltransferase activity (Probable).
Protein Name Arginine-Hydroxylase Ndufaf5 - Mitochondrial
Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 5
Putative Methyltransferase Ndufaf5
Database Links Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripherally Localized On The Matrix Face Of The Mitochondrial Inner Membrane
Alternative Antibody Names Anti-Arginine-Hydroxylase Ndufaf5 - Mitochondrial antibody
Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 5 antibody
Anti-Putative Methyltransferase Ndufaf5 antibody
Anti-NDUFAF5 antibody
Anti-C20orf7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance