• Immunofluorescence analysis of paraffin-embedded mouse bone marrow using TPO-R/CD110/c-TPO-R/CD110/c-Mpl Rabbit polyclonal antibody (STJ119126) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded human spleen using TPO-R/CD110/c-TPO-R/CD110/c-Mpl Rabbit polyclonal antibody (STJ119126) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat bone marrow using TPO-R/CD110/c-TPO-R/CD110/c-Mpl Rabbit polyclonal antibody (STJ119126) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse heart, using TPO-R/CD110/c-Mpl Rabbit polyclonal antibody (STJ119126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MPL antibody (295-390) (STJ119126)

SKU:
STJ119126

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MPL
Gene ID: 4352
Uniprot ID: TPOR_HUMAN
Immunogen Region: 295-390
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 295-390 of human TPO-R/CD110/c-Mpl (NP_005364.1).
Immunogen Sequence: DLKNVTCQWQQQDHASSQGF FYHSRARCCPRDRYPIWENC EEEEKTNPGLQTPQFSRCHF KSRNDSIIHILVEVTTAPGT VHSYLGSPFWIHQAVR
Tissue Specificity Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed.
Post Translational Modifications Phosphorylated at Tyr-591 in response to THPO stimulation. Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination.
Function Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K. In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production.
Protein Name Thrombopoietin Receptor
Tpo-R
Myeloproliferative Leukemia Protein
Proto-Oncogene C-Mpl
Cd Antigen Cd110
Database Links Reactome: R-HSA-76009
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Cell Surface
Alternative Antibody Names Anti-Thrombopoietin Receptor antibody
Anti-Tpo-R antibody
Anti-Myeloproliferative Leukemia Protein antibody
Anti-Proto-Oncogene C-Mpl antibody
Anti-Cd Antigen Cd110 antibody
Anti-MPL antibody
Anti-TPOR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance