| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Lens fiber major intrinsic protein (95-144 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MIP |
| Gene ID: | 4284 |
| Uniprot ID: | MIP_HUMAN |
| Immunogen Region: | 95-144 aa |
| Specificity: | AQP0 Polyclonal Antibody detects endogenous levels of AQP0 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human AQP0 at the amino acid range 95-144 |
| Post Translational Modifications | Subject to partial proteolytic cleavage in the eye lens core. Partial proteolysis promotes interactions between tetramers from adjoining membranes. Fatty acylated at Met-1 and Lys-238. The acyl modifications, in decreasing order of ion abundance, are: oleoyl (C18:1) > palmitoyl (C16:0) > stearoyl (C18:0) > eicosenoyl (C20:1) > dihomo-gamma-linolenoyl (C20:3) > palmitoleoyl (C16:1) > eicosadienoyl (C20:2). |
| Function | Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Specifically expressed in lens fiber cells, this aquaporin is crucial for maintaining lens water homeostasis and transparency. Beyond water permeability, it also acts as a cell-to-cell adhesion molecule, forming thin junctions between lens fiber cells that are essential for maintaining the ordered structure and transparency of the lens. |
| Protein Name | Lens Fiber Major Intrinsic Protein Aquaporin-0 Mip26 Mp26 |
| Database Links | Reactome: R-HSA-432047 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Cell Junction Localizes To Thin Cell-Cell Junctions In Lens Fiber Cells |
| Alternative Antibody Names | Anti-Lens Fiber Major Intrinsic Protein antibody Anti-Aquaporin-0 antibody Anti-Mip26 antibody Anti-Mp26 antibody Anti-MIP antibody Anti-AQP0 antibody |
Information sourced from Uniprot.org